Composite

Part:BBa_K389413:Design

Designed by: Jonas Aretz   Group: iGEM10_Bielefeld-Germany   (2010-10-11)

vir promoter + sensitivity tuner + luciferase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2455
    Illegal NgoMIV site found at 3799
    Illegal NgoMIV site found at 3820
    Illegal AgeI site found at 1978
    Illegal AgeI site found at 2090
    Illegal AgeI site found at 2292
    Illegal AgeI site found at 3523
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 3705


Design Notes

a modified sensitivity tuner BBa_I746390 to enhance the luciferase signal which interacts as a reporter for a vir promoter

Source

References

[http://2007.igem.org/Cambridge/Amplifier_project Cambridge iGEM Team wiki 2007, amplifier project]